Dragon fruit leaves extract contains various phytochemicals, namely: alkaloids, flavonoids, tannins, saponins, glycosides, steroids, and terpenoids. Diverse phytochemical content allows the dragon fruit leaves extract to have antibacterial activity. The objectives of this research were to test antibacterial activity of dragon fruit leaves extract against meningitis bacterial (Neisseria meningitidis, Streptococcus pneumoniae, and Listeria monocytogenes). Dragon fruit leaves extract was obtained by maceration. Antibacterial activity test was done by the paper disc diffusion method with ceftriaxone as the positive control and dimethylsulfoxide as the negative control. Antibacterial activity of red dragon leaves extract with concentration 600 μg/mL and white dragon fruit leaves extract with concentration 800 μg/mL were not show significantly different antibacterial activity from ceftriaxone with concentration 10 μg/mL against meningitis bacterial (Neisseria meningitidis, Streptococcus pneumoniae, and Listeria monocytogenes). The results prove that the red dragon fruit leaves extract was more potential antibacterial activity than white dragon fruit leaves extracted against meningitis bacterial (Neisseria meningitidis, Streptococcus pneumoniae, and Listeria monocytogenes).