Medicinal plants are widely available and affordable, especially in rural areas. Investigation into medicinal plants has shown that they have significant antiproliferative (cancer prevention) and antibacterial properties, which are attributed in part to bioactive components. These compounds' mechanisms of action, potential therapeutic applications, and absence of side effects in comparison to synthetic pharmaceuticals have sparked attention in the scientific literature. Therefore, these medicinal herbs are utilised for creating formulations that may serve as supplements to improve general health, such as antioxidants and immune boosters. In view of this, we characterize formulations of Zanthoxylum armatum (fruit and seed) using GC-MS from various locations in Kumaun, Uttarakhand, and assess their antimicrobial properties (including MIC and MBC determination) and inhibition of the growth (anti-proliferative activity) of two human cancer cell lines (SW-480 and HeLa) and one healthy cell line (KMST-6). These research' findings demonstrated that the formulation (fruit/seed; 40:60) inhibited the growth of two different cancer cell lines (HeLa and SWS 480) and showed less inhibition in the case of the normal cell line, i.e., KMST-6. Similarly, this formulation demonstrated antimicrobial activities when measured against minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). In short, this formulation has higher antiproliferative and antimicrobial properties in most of the assay when tested individually, as compared to the rest of the formulation. Thus, it is sensible to use the combination of studied herbal formulations for the development of any antimicrobial supplement, food product, or anticancer molecule in the future.