Bacterial cellulose (BC), a polysaccharide produced by bacteria, is widely used in various fields. In our previous study, we isolated a BCproducing bacterium, Komagataeibacter sp. MI 2, and investigated its ability to produce BC. However, the genetic information of this bacterium has not yet been investigated. Therefore, the aim of this study was to analyze the BC-producing Komagataeibacter sp. MI 2 using whole genome sequencing technologies. The bacterial genome was assembled from both short- and long-reads data and yielded 4 completed circular chromosomes consisting of 1 chromosome and 3 plasmids. A total of 3.8 Mb of the genome was 99% assembled completeness analyzed by BUSCO, 60.28% of GC content, and 3.52 Mb of N50. Among the 3,612 annotated genes, most genes play a role in metabolic and secondary metabolite biosynthetic pathways according to KEGG analysis. Comparative genomic analysis revealed that Komagataeibacter sp. MI 2 is very related to K. diospyri indicating that it is most likely K. diospyri MI 2. We found a putative bacteriocin, linocin M18, and no antibiotic-resistant gene was identified in the genome of K. diospyri MI 2. In addition, several genetic mobile elements were identified in the genome. For BC biosynthesis, we compared the bsc operon and BC biosynthesis genes between Komagataeibacter sp. The result showed that most Komagataeibacter sp., including K. diospyri MI 2, contained almost all BC biosynthesis-related genes in the genome, suggesting potentially BC-producing bacteria. This study provided genomic information of K. diospyri MI 2 and its potential for bacterial cellulose production.