The purpose of this study was to investigate nurses’ and nursing students’ knowledge and perceived role in assisting patients with the nutritional management of diabetes. Three focus groups were conducted and the results were used to modify a previously developed survey regarding the nutritional management of diabetes. The survey was administered via an online survey tool and completed by 231 nurses and students. Over 70% of respondents agreed that nurses have an important role in reinforcing patient nutritional education. Results indicated, however, that knowledge gaps in the nutritional management of diabetes exist among nurses, including not knowing the carbohydrate content of 120 ml of orange juice, a common treatment for hypoglycaemia (47.5%), not knowing where to locate carbohydrate content on a food label (60%), and not identifying the correct treatment for hypoglycaemia (47.5%). These results indicate that there may be a need to improve the nutritional education of nurses with respect to diabetes management.