The family Phyllostomidae includes the species Phyllostomus hastatus, which is distinguished by its large size and wide distribution; it is an omnivorous bat that feeds mainly on invertebrates and fruits. However, there are reports of predation on small vertebrates, such as bats, of which the cases documented in the scientific literature are usually few. Using 2 5-ring mist nets, 12 m long by 2.5 m wide, placed mainly at ground level on the banks of the Pedro Miguel River, in an area of secondary tropical lowland vegetation, captures were made by monitoring the abundance and richness of bats in Camino de Cruces National Park. On July 30, 2024, in Camino de Cruces National Park, Panamá, we reported a predation attempt by P. hastatus on Artibeus jamaicensis. To the best of our knowledge, this constitutes the first predator-prey interaction between both bats and highlights the opportunistic role of P. hastatus as a predator.