Expression of Envelope Protein (E2) of Bovine Viral Diarrhea Virus in Insect Cells.

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1997 English Publisher:Japanese Society of Veterinary ScienceJournal:Journal of Veterinary Medical Science, volume 59, pages 415-419 (issn: 0916-7250, eissn: 1347-7439,

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Authors: C H, Kweon; Y D, Yoon; S H, An; Y S, Lee;

doi: 10.1292/jvms.59.415

pmid: 9192369

Expression of Envelope Protein (E2) of Bovine Viral Diarrhea Virus in Insect Cells.

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Abstract

The gene encoding the envelope glycoprotein (E2) of bovine viral diarrhea virus (BVDV) was expressed in a baculovirus. The expressed protein was detected on the surface of infected cells by immunofluorescence. Western blotting analysis showed the presence of the expressed protein of a similar molecular size to the E2 protein. The antigenicity of expressed protein were tested in guinea pigs and cattle. The immunized animals developed neutralizing antibodies against BVDV.

Related Organizations

Seoul National University
Korea (Republic of)
Rural Development Administration
Korea (Republic of)
National Veterinary Research Institute
Nigeria

Keywords

Genetic Vectors, Guinea Pigs, Spodoptera, Antibodies, Viral, Transfection, Recombinant Proteins, Cell Line, Viral Envelope Proteins, Pestivirus, Animals, Bovine Virus Diarrhea-Mucosal Disease, Cattle, Baculoviridae

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