Both the upper and lower temperature tolerances of red fluorescent protein transgenic zebra danios and wildtype zebra danios, Danio rerio, were significantly different via two different methods; however, all differences are small (< 1°C) and probably not ecologically important. The U.S. geographic distributions of both transgenic and wildtype zebra danios will not be restricted by their upper thermal tolerances, but will be limited to the southern and western portions of the U.S. by their lower thermal tolerances. Largemouth bass did not preferentially prey upon transgenic zebra danios compared to wildtype danios or wildtypes relative to a native fish. If transgenic or wildtype zebra danios are released into southern or western U.S. waters, it is possible they could be eliminated by predation.