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2. Histocytochemistry Series A New Antigen Retrieval Method (6)

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2010 English Publisher:Medical Association of Nippon Medical SchoolJournal:Nihon Ika Daigaku Igakkai Zasshi, volume 6, pages 178-184 (issn: 1349-8975, eissn: 1880-2877,

Authors: Shigeki Namimatsu; Yuichi Sugisaki; Shinichi Tsuchiya;

doi: 10.1272/manms.6.178

2. Histocytochemistry Series A New Antigen Retrieval Method (6)

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Abstract

Current antigen retrieval techniques include the use of citrate buffer, Tris-HCl containing 5% urea, and EDTA solutions combined with heating in a microwave oven or autoclave. These methods must be adjusted for a given tissue or antigen. To improve the efficiency of antigen retrieval for immunohistochemical staining, we developed a new method using citraconic anhydride. We describe this new antigen retrieval method using 0.05% citraconic anhydride solution of pH 7.4 and heat. This antigen retrieval method produced satisfactory staining results for a wide variety of antigens.

Related Organizations

Nippon Medical School Hospital
Japan

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