Cloning and Expression Analysis of Cathepsin D in the Olive FlounderParalichthys olivaceus
Copyright policy )Cloning and Expression Analysis of Cathepsin D in the Olive FlounderParalichthys olivaceus
We isolated a homolog of cathepsin D from the cDNA library of the olive flounder kidney. The olive flounder cathepsin D transcript consisted of 1,733 bp that encoded a polypeptide of 396 amino acids. The overall similarity between olive flounder cathepsin D and other cathepsin Ds was very high, with the highest amino acid sequence identity to barramundi perch (89%). RT-PCR revealed that cathepsin D was expressed in almost all tissues, with high expression in the liver, intestine, kidney, skin, and spleen. The accumulation of cathepsin D mRNA after bacterial infection, as determined by RT-PCR, was constitutive and increased greatly after bacterial infection.
- Korea Research Institute of Bioscience and Biotechnology Korea (Republic of)
- National Fisheries Research and Development Institute Korea (Republic of)
- National Fisheries Research & Development Institute
<I>Paralichthys olivaceus</I>, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Molecular Sequence Data, cathepsin D, RT-PCR, Flounder, Cathepsin D, Dogs, Gene Expression Regulation, gene expression, Animals, Humans, Cattle, Amino Acid Sequence, Cloning, Molecular, Sequence Alignment, Phylogeny
<I>Paralichthys olivaceus</I>, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Molecular Sequence Data, cathepsin D, RT-PCR, Flounder, Cathepsin D, Dogs, Gene Expression Regulation, gene expression, Animals, Humans, Cattle, Amino Acid Sequence, Cloning, Molecular, Sequence Alignment, Phylogeny
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