Structural Analysis of a Bioactive Polysaccharide, PISP1, from the Medicinal MushroomPhellinus igniarius

descriptionPublicationkeyboard_double_arrow_right Article 23 Jan 2009 English Publisher:Oxford University Press (OUP)Journal:Bioscience, Biotechnology, and Biochemistry, volume 73, pages 134-139 (issn: 0916-8451, eissn: 1347-6947,

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Authors: YANG, Yan; YE, LiBin; ZHANG, JingSong; LIU, YanFang; TANG, QingJiu;

doi: 10.1271/bbb.80546

pmid: 19129637

Structural Analysis of a Bioactive Polysaccharide, PISP1, from the Medicinal MushroomPhellinus igniarius

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Abstract

PISP1, a heteropolysaccharide isolated from fruiting bodies of Phellinus igniarius by hot aqueous extraction and purified by DEAE-Sepharose anion-exchange and gel filtration chromatography, is composed of fucose, galactose, mannose, and 3-O-Me-galactose in a ratio of 1:2:1:2. Methylation, monosaccharide analysis, and NMR studies (1H NMR, 13C NMR, COSY-45 degrees , TOCSY, ROESY, HSQC, and HMBC) revealed that PISP1 had a backbone consisting of 1,6-disubstituted-3-O-Me-alpha-D-galactopyranosyl residue, 1,3,6-trisubstituted-alpha-D-manopyranosyl residue, 1,4-disubstituted-alpha-D-galactopyranosyl residue, and 1,2-disubstituted-alpha-D-galactopyranosyl residue, and had a 1-substituted-alpha-L-fucopyranosyl terminal attached to O-3 of a manopyranosyl residue. Preliminary bioactivity tests conducted in vitro revealed that PISP1 stimulated the proliferation of mouse spleen lymphocytes.

Related Organizations

Zhejiang Gongshang University
China (People's Republic of)
Shanghai Academy of Agricultural Sciences
China (People's Republic of)

Keywords

Molecular Structure, heteropolysaccharide, Monosaccharides, <I>Phellinus igniarius</I>, Mice, NMR spectroscopy, bioactivity, Polysaccharides, Animals, structural analysis, Fruiting Bodies, Fungal, Lymphocytes, Agaricales, Spleen, Cell Proliferation

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