Yeast Cell-Surface Expression of Chitosanase fromPaenibacillus fukuinensis
Copyright policy )Yeast Cell-Surface Expression of Chitosanase fromPaenibacillus fukuinensis
To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo assay and the dinitrosalicylic acid method.
- Fukui Prefectural University Japan
- University of Fukui Japan
- Kyoto University Japan
- Fukui University of Technology Japan
Glycoside Hydrolases, Cell Membrane, Oligosaccharides, Bacillus, Saccharomyces cerevisiae, Catalysis, Recombinant Proteins, Bacterial Proteins, Cloning, Molecular, Genetic Engineering, Biotechnology, Plasmids
Glycoside Hydrolases, Cell Membrane, Oligosaccharides, Bacillus, Saccharomyces cerevisiae, Catalysis, Recombinant Proteins, Bacterial Proteins, Cloning, Molecular, Genetic Engineering, Biotechnology, Plasmids
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).19 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!