The Role of the DRY Motif of Human MC4R for Receptor Activation
Copyright policy )The Role of the DRY Motif of Human MC4R for Receptor Activation
We constructed several mutant human MC4R cDNAs by site directed mutagenesis and expressed these receptors in COS-1 cells. The conserved DRY motif among GPCRs was mutated to generate eight mutants. While no MC4R ligand binding was detected in any of the mutants, one mutant, D146A, resulted in higher cAMP production in cells than the wild-type receptor without ligand stimulation.
- Taisho Pharmaceutical (Japan) Japan
- Tottori University Japan
melanocortin type 4 receptor, Amino Acid Motifs, Mutation, Missense, DRY motif, Ligands, Transfection, Receptors, G-Protein-Coupled, site directed mutagenesis, cAMP, G-protein coupled receptor, COS Cells, Cyclic AMP, Mutagenesis, Site-Directed, Animals, Humans, Receptor, Melanocortin, Type 4, Amino Acid Sequence, Protein Binding
melanocortin type 4 receptor, Amino Acid Motifs, Mutation, Missense, DRY motif, Ligands, Transfection, Receptors, G-Protein-Coupled, site directed mutagenesis, cAMP, G-protein coupled receptor, COS Cells, Cyclic AMP, Mutagenesis, Site-Directed, Animals, Humans, Receptor, Melanocortin, Type 4, Amino Acid Sequence, Protein Binding
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