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Bioscience Biotechnology and Biochemistry
Article . 2003 . Peer-reviewed
Full-Text: http://www.jstage.jst.go.jp/article/bbb/67/7/67_7_1465/_pdf
Data sources: Japan Link Center
Bioscience Biotechnology and Biochemistry
Article . 2003 . Peer-reviewed
Data sources: Crossref
Bioscience Biotechnology and Biochemistry
Article . 2004
Data sources: Europe PubMed Central
https://dx.doi.org/10.1271/bbb...
Article
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https://dx.doi.org/10.1271/bbb...
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Production of Galactinol from Sucrose by Plant Enzymes

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2003 English Publisher:Oxford University Press (OUP)Journal:Bioscience, Biotechnology, and Biochemistry, volume 67, pages 1,465-1,471 (issn: 0916-8451, eissn: 1347-6947, Copyright policy )
Authors: WAKIUCHI, Nariaki; SHIOMI, Ryohei; TAMAKI, Hajime;

doi: 10.1271/bbb.67.1465

pmid: 12913288

Production of Galactinol from Sucrose by Plant Enzymes

Abstract

Galactinol, 1-O-(alpha-D-galactopyranosyl)-myo-inositol, was produced from sucrose as a starting material. UDP-Glc was prepared with sucrose and UDP using sucrose synthase partially purified from sweet potato roots. Then, the UDP-Glc was converted to UDP-Gal using yeast UDP-Gal 4-epimerase from a commercial source. Finally, galactinol was produced from the UDP-Gal and myo-inositol using galactinol synthase partially purified from cucumber leaves. The product was identified as galactinol by the retention times of HPLC, alpha-galactosidase digestion, and NMR spectrometry.

Related Organizations
Keywords

Sucrose, Magnetic Resonance Spectroscopy, Disaccharides, Galactosyltransferases, galactinol synthase, UDP-Galactose 4-epimerase, Glucosyltransferases, cucumber leaves, Cucumis sativus, Ipomoea batatas, galactinol, sucrose synthase, Chromatography, High Pressure Liquid

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    popularity
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    influence
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    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
4
Average
Average
Average
bronze