Transglycosylation of Glycosyl Residues to Cyclic Tetrasaccharide byBacillus stearothermophilusCyclomaltodextrin Glucanotransferase Using Cyclomaltodextrin as the Glycosyl Donor
Copyright policy )Transglycosylation of Glycosyl Residues to Cyclic Tetrasaccharide byBacillus stearothermophilusCyclomaltodextrin Glucanotransferase Using Cyclomaltodextrin as the Glycosyl Donor
Cyclomaltodextrin glucanotransferase (EC 2.4.1.19, abbreviated as CGTase) derived from Bacillus stearothermophilus produced a series of transfer products from a mixture of cyclomaltohexaose and cyclic tetrasaccharide (cyclo[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->], CTS). Of the transfer products, only two components, saccharides A and D, remained and accumulated after digestion with glucoamylase. The total combined yield of the saccharides reached 63.4% of total sugars, and enzymatic and instrumental analyses revealed the structures of both saccharides. Saccharide A was identified as 4-mono-O-alpha-glucosyl-CTS, [-->6)-[alpha-D-Glcp-(1-->4)]-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->], and sachharide D was 4,4'-di-O-alpha-glucosyl-CTS, [-->6)-[alpha-D-Glcp-(1-->4)]-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-[alpha-D-Glcp-(1-->4)]-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->]. These structures led us to conclude that the glycosyltransfer catalyzed by CGTase was specific to the C4-OH of the 6-linked glucopyranosyl residues in CTS.
Glycosylation, Hydrolysis, Oligosaccharides, Bacillus, alpha-Glucosidases, beta-Amylase, Methylation, Catalysis, Mass Spectrometry, Geobacillus stearothermophilus, Glucosyltransferases, Polysaccharides, Aspergillus niger, Glycosides, Glucan 1,4-alpha-Glucosidase, Chromatography, High Pressure Liquid
Glycosylation, Hydrolysis, Oligosaccharides, Bacillus, alpha-Glucosidases, beta-Amylase, Methylation, Catalysis, Mass Spectrometry, Geobacillus stearothermophilus, Glucosyltransferases, Polysaccharides, Aspergillus niger, Glycosides, Glucan 1,4-alpha-Glucosidase, Chromatography, High Pressure Liquid
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