Biotransformation of (−)-Verbenone by Human Liver Microsomes

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2002 English Publisher:Informa UK LimitedJournal:Bioscience, Biotechnology, and Biochemistry, volume 66, pages 2,458-2,460 (issn: 0916-8451, eissn: 1347-6947,

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Authors: Mitsuo, Miyazawa; Atsushi, Sugie; Masaki, Shindo;

doi: 10.1271/bbb.66.2458

pmid: 12506987

Biotransformation of (−)-Verbenone by Human Liver Microsomes

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Abstract

The biotransformation of (-)-verbenone was investigated with human liver microsomes by using GC-MS. Regioselective biotransformation was observed when (-)-verbenone was incubated with the liver microsomes. (-)-10-Hydroxyverbenone was formed from (-)-verbenone of kinetic analysis showed that the Km and Vmax values for the hydroxylation of (-)-verbenone by liver microsomes from three human samples, HG-70, HG-56 and HG-23, were 1.1 mM and 4.8 nmol/min/nmol P450, 0.6 mM and 2.1 nmol/min/nmol P450, and 2.8 mM and 4.6 nmol/min/nmol P450, respectively.

Related Organizations

Kindai University
Japan

Keywords

Terpenes, Stereoisomerism, Hydroxylation, Gas Chromatography-Mass Spectrometry, Rosmarinus, Kinetics, Microsomes, Liver, Humans, Biotransformation, Bicyclic Monoterpenes

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