Transglycosylation to Ginseng Saponins by Cyclomaltodextrin Glucanotransferases
Copyright policy )Transglycosylation to Ginseng Saponins by Cyclomaltodextrin Glucanotransferases
Ten new alpha-glucosylginsenosides were found to be synthesized from dextrin and four ginsenosides, -Rb1, -Rc, -Re, and -Rg1, by the successive actions of B. stearothermophilus cyclomaltodextrin glucanotransferase and Rhizopus glucoamylase. Seven of them were isolated in the pure state by extraction with n-butanol saturated with water, silica gel column chromatography, and high pressure liquid chromatography, and identified as 3-O-[alpha-D-glcp-(1-->4)-beta-D-glcp-(1-->2)-beta-D-glcp]-20-O-[beta-D-glcp-(1-->6)-beta-D-glcp]-20(S)-protopanaxadiol, 3-O-[beta-D-glcp-(1-->2)-beta-D-glcp]-20-O-[alpha-D-glcp-(1-->4)-beta-D-glcp-(1-->6)-beta-D-glcp]-20(S)-protopanaxadiol, 3-O-[alpha-D-glcp-(1-->4)-beta-D-glcp-(1-->2)-beta-D-glcp]-20-O-[alpha-L-araf-(1-->6)-beta-D-glcp]-20(S)-protopanaxadiol, 3-O-[beta-D-glcp-(1-->2)-beta-D-glcp]-20-O-[(4G-alpha-D-glcp)-alpha-L-araf-(1-->6)-beta-D-glcp]-20(S)-protopanaxadiol, 6-O-[alpha-L-rhap-(1-->2)-beta-D-glcp]-20-O-[alpha-D-glcp-(1-->4)-beta-D-glcp]-20(S)-protopanaxatriol, 6-O-[alpha-D-glcp-(1-->4)-beta-D-glcp]-20-O-(beta-D-glcp)-20(S)-protopanaxatriol, and 6-O-[alpha-D-glcp-(1-->3)-beta-D-glcp]-20-O-(beta-D-glcp)-20(S)-protopanaxatriol, by spectroscopy (FAB-MS, IR, 1H-NMR and 13C-NMR) and hydrolysis products in 50% acetic acid. The bitterness of these alpha-glucosyl-ginsenosides was less than that of ginsenosides.
- Okayama University Japan
Magnetic Resonance Spectroscopy, Plants, Medicinal, α-glucosylginsenoside Rg<SUB>1</SUB>, Hydrolysis, Panax, Saponins, Spectrometry, Mass, Fast Atom Bombardment, α-glucosylginsenoside Rc, Plant Roots, α-glucosylginsenoside Rb<SUB>1</SUB>, α-glucosylginsenoside Re, Bacillus stearothermophilus cyclomaltodextrin glucanotransferase, Glucosyltransferases, Chromatography, Thin Layer, Chromatography, High Pressure Liquid
Magnetic Resonance Spectroscopy, Plants, Medicinal, α-glucosylginsenoside Rg<SUB>1</SUB>, Hydrolysis, Panax, Saponins, Spectrometry, Mass, Fast Atom Bombardment, α-glucosylginsenoside Rc, Plant Roots, α-glucosylginsenoside Rb<SUB>1</SUB>, α-glucosylginsenoside Re, Bacillus stearothermophilus cyclomaltodextrin glucanotransferase, Glucosyltransferases, Chromatography, Thin Layer, Chromatography, High Pressure Liquid
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