A Simple, Rapid, and Highly Efficient Gene Expression System for Multiheme Cytochromes c
Copyright policy )A Simple, Rapid, and Highly Efficient Gene Expression System for Multiheme Cytochromes c
The genes of tetraheme cytochrome c3 and hexadecaheme high-molecular-weight cytochrome c from Desulfovibrio vulgaris could be overexpressed as holoproteins in Shewanella oneidensis TSP-C using pUC-type vectors of E. coli. Surprisingly, S. oneidensis was transformed directly by pUC-type vectors through electroporation. The yields of the recombinant proteins in this expression system were much higher than the previously reported ones.
- Yokohama National University Japan
- Osaka University Japan
electroporation, Shewanella, Magnetic Resonance Spectroscopy, pUC-type vector, Genetic Vectors, multiheme cytochrome c, Gene Expression, Cytochrome c Group, Recombinant Proteins, Shewanella oneidensis, Electrophoresis, Polyacrylamide Gel, Oxidation-Reduction
electroporation, Shewanella, Magnetic Resonance Spectroscopy, pUC-type vector, Genetic Vectors, multiheme cytochrome c, Gene Expression, Cytochrome c Group, Recombinant Proteins, Shewanella oneidensis, Electrophoresis, Polyacrylamide Gel, Oxidation-Reduction
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