A Simple, Rapid, and Highly Efficient Gene Expression System for Multiheme Cytochromes c
Copyright policy )A Simple, Rapid, and Highly Efficient Gene Expression System for Multiheme Cytochromes c
The genes of tetraheme cytochrome c3 and hexadecaheme high-molecular-weight cytochrome c from Desulfovibrio vulgaris could be overexpressed as holoproteins in Shewanella oneidensis TSP-C using pUC-type vectors of E. coli. Surprisingly, S. oneidensis was transformed directly by pUC-type vectors through electroporation. The yields of the recombinant proteins in this expression system were much higher than the previously reported ones.
- Yokohama National University Japan
- Osaka University Japan
Shewanella, Magnetic Resonance Spectroscopy, Genetic Vectors, Gene Expression, Cytochrome c Group, Electrophoresis, Polyacrylamide Gel, Oxidation-Reduction, Recombinant Proteins
Shewanella, Magnetic Resonance Spectroscopy, Genetic Vectors, Gene Expression, Cytochrome c Group, Electrophoresis, Polyacrylamide Gel, Oxidation-Reduction, Recombinant Proteins
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