Estimation of the Number of Polyhydroxyalkanoate (PHA)-Degraders in Soil and Isolation of Degraders Based on the Method of Most Probable Number (MPN) Using PHA-Film

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2001 English Publisher:Oxford University Press (OUP)Journal:Bioscience, Biotechnology, and Biochemistry, volume 65, pages 1,214-1,217 (issn: 0916-8451, eissn: 1347-6947,

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Authors: SONG, Cunjiang; UCHIDA, Urumi; ONO, Shin; SHIMASAKI, Choichiro; INOUE, Masami;

doi: 10.1271/bbb.65.1214

pmid: 11440143

Estimation of the Number of Polyhydroxyalkanoate (PHA)-Degraders in Soil and Isolation of Degraders Based on the Method of Most Probable Number (MPN) Using PHA-Film

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Abstract

A new method to estimate the number of polyhydroxyalkanoates (PHA)-degraders in soil and to isolate degraders, called the film-MPN method, is proposed. The incubation time was measured by the first order reaction (FOR) model. This method was used to estimate numbers of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)[P(3HB-co-3HV)]- and poly(3-hydroxyvalerate-co-4-hydroxybutyrate)[P(3HB-co-4HB)]-degraders in garden soil (4.30 x 10(5) and 2.15 x 10(5) aerobic degraders per gram of dry soil, respectively). The number of P(3HB-co-3HV)-degraders in paddy field soil was 5.06 x 10(5) aerobic degraders per gram dry soil. Also, several P(3HB-co-3HV)-degraders were isolated directly from positive-growth tubes of high dilution.

Related Organizations

University of Toyama
Japan

Keywords

P(3HB-co-3HV), PHA, Colony Count, Microbial, biodegradation, P(3HB-co-4HB), Biodegradation, Environmental, film-MPN, Biofilms, Polycyclic Compounds, Soil Microbiology

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