Binding Affinity of the Methyl Ester of AK-toxin I to Membrane Fractions from Japanese Pear Leaves
Copyright policy )Binding Affinity of the Methyl Ester of AK-toxin I to Membrane Fractions from Japanese Pear Leaves
The binding site of AK-toxin, a host-specific toxin against Japanese pear, was searched for in the membrane fractions of the pear leaves, using 3H-labeled AK-toxin I methyl ester. Binding activity, which was displaceable by the unlabeled ligand, was observed for microsomal fraction from a toxin-susceptible cultivar, Nijisseiki. However, the binding was also observed for those from toxin-resistant cultivars, Kosui and Hosui. Detection of the specific binding failed for the plasma membrane fraction which was prepared from microsomal fraction of the toxin-susceptible cultivar by aqueous two-phase separation, and the hitherto presumed model of the AK-toxin receptor in the plasma membrane could not be verified.
- Kyoto University Japan
Plant Leaves, Microsomes, Phenylalanine, Cell Membrane, Fatty Acids, Unsaturated, Mycotoxins, Rosales, Binding, Competitive
Plant Leaves, Microsomes, Phenylalanine, Cell Membrane, Fatty Acids, Unsaturated, Mycotoxins, Rosales, Binding, Competitive
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