descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1997 English Publisher:Oxford University Press (OUP)Journal:Bioscience, Biotechnology, and Biochemistry, volume 61, pages 1,955-1,956 (issn: 0916-8451, eissn: 1347-6947,

Authors: Takahisa Hayashi; Tomonori Nakai; Hitoshi Mori; Fukumi Sakai; Naoto Tonouchi; Takayasu Tsuchida;

doi: 10.1271/bbb.61.1955

pmid: 9404082

Synthesis of Asymmetrically Labeled Sucrose by a Recombinant Sucrose Synthase

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Abstract

About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[14C]glucose or [14C]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose synthase has a similar affinity for UDP-glucose and fructose to an intact enzyme from the mung bean, but a lower affinity for sucrose.

Related Organizations

Nagoya University
Japan
Kyoto University
Japan
Tokai National Higher Education and Research System
Japan

Keywords

Uridine Diphosphate Glucose, Sucrose, Glucosyltransferases, Isotope Labeling, Escherichia coli, Carbon Radioisotopes, Fructose, Recombinant Proteins

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