A Ribosome–Inactivating Protein fromAmaranthus viridis

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1997 English Publisher:Oxford University Press (OUP)Journal:Bioscience, Biotechnology, and Biochemistry, volume 61, pages 1,613-1,614 (issn: 0916-8451, eissn: 1347-6947,

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Authors: KWON, Seok-Yoon; AN, Chung Sun; LIU, Jang Ryol; PAEK, Kyung-Hee;

doi: 10.1271/bbb.61.1613

pmid: 9339569

A Ribosome–Inactivating Protein fromAmaranthus viridis

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Abstract

An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus (TMV) infection test on Nicotiana glutinosa leaves. The molecular mass of the amaranthin was estimated about 30 kDa by SDS-PAGE and the pI was measured as 9.8 by isoelectric focusing (IEF) analysis. Cytotoxicity of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) with IC50 of 25 pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.

Related Organizations

Seoul National University
Korea (Republic of)
Korea Research Institute of Bioscience and Biotechnology
Korea (Republic of)
Korea University
Korea (Republic of)

Keywords

Amaranthus viridis, Plants, Medicinal, Reticulocytes, antiviral protein, Chromatography, Ion Exchange, Antiviral Agents, in vitro translation, TMV, Molecular Weight, Plant Leaves, Tobacco Mosaic Virus, RIP, Ribosome Inactivating Proteins, Type 1, Animals, Electrophoresis, Polyacrylamide Gel, Rabbits, Isoelectric Focusing, N-Glycosyl Hydrolases, Plant Proteins

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