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Bioscience Biotechnology and Biochemistry
Article . 1997 . Peer-reviewed
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A Conjugative Linear Plasmid inStreptomyces laurentiiATCC31255

Authors: KINOSHITA-IRAMINA, Chizuru; KITAHARA, Maki; DOI, Katsumi; OGATA, Seiya;

A Conjugative Linear Plasmid inStreptomyces laurentiiATCC31255

Abstract

Plasmid pSLL of Streptomyces laurentii ATCC31255 (wild-type strain P0) is a 93-kilobase linear DNA plasmid that carries a protein bound to each 5' end of the DNA. It was self-transmitted to the pSLL-cured strain by conjugation in solid culture. The pSLL-cured strain carried a circular plasmid, pSLS, and showed a marked decrease in spore formation and thiostrepton productivity, owing to the pSLS. However, by retransmission of pSLL, these things reverted to levels seen in strain P0. Thus, plasmid pSLL suppressed the injurious effects of pSLS on the host mycelia.

Related Organizations
Keywords

Exonucleases, Spores, Bacterial, Restriction Mapping, Thiostrepton, Streptomyces, Anti-Bacterial Agents, Culture Media, Blotting, Southern, conjugative plasmid, Conjugation, Genetic, Electrophoresis, Polyacrylamide Gel, linear plasmid, Plasmids

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
6
Average
Average
Average