Solubilizing Coelenterazine in Water with Hydroxypropyl-β-cyclodextrin

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1997 English Publisher:Informa UK LimitedJournal:Bioscience, Biotechnology, and Biochemistry, volume 61, pages 1,219-1,220 (issn: 0916-8451, eissn: 1347-6947,

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Authors: TERANISHI, Katsunori; SHIMOMURA, Osamu;

doi: 10.1271/bbb.61.1219

Solubilizing Coelenterazine in Water with Hydroxypropyl-β-cyclodextrin

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Abstract

Coelenterazine is poorly soluble in water except at alkaline pH values that promote auto-oxidation. The solubility is markedly increased by adding hydroxypropyl-β-cyclodextrin in a neutral buffer solution, the solubilization being most effective in the pH range of 6.5 to 7.0. The concentration of coelenterazine dissolved in a pH 7.0 buffer containing 50 mm hydroxypropyl-β-cyclodextrin was about 280-fold higher than that without the additive.

Related Organizations

Marine Biological Laboratory
United States

Keywords

coelenterazine, solubility, hydroxypropyl-β-cyclodextrin

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