Synthesis of Glycosyl-trehaloses by Cyclomaltodextrin Glucanotransferase through the Transglycosylation Reaction

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1997 English Publisher:Oxford University Press (OUP)Journal:Bioscience, Biotechnology, and Biochemistry, volume 61, pages 1,146-1,149 (issn: 0916-8451, eissn: 1347-6947,

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Authors: M, Kurimoto; A, Tabuchi; T, Mandai; T, Shibuya; H, Chaen; S, Fukuda; T, Sugimoto; +1 Authors

doi: 10.1271/bbb.61.1146

pmid: 9255978

Synthesis of Glycosyl-trehaloses by Cyclomaltodextrin Glucanotransferase through the Transglycosylation Reaction

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Abstract

Cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus produced a series of glycosyl-trehaloses through the transglycosylation reaction with cyclomaltohexaose as the glycosyl donor and trehalose as its acceptor. After beta-amylase treatment, five species of glycosyl-trehaloses were isolated by column chromatography. After chemical and enzymatic analyses, it was concluded that these oligosaccharides were alpha-maltosyl alpha-D-glucopyranoside, alpha-maltotriosyl alpha-D-glucopyranoside, alpha-maltosyl alpha-maltoside, alpha-maltotriosyl alpha-maltoside, and alpha-maltotriosyl alpha-maltotrioside. These were not hydrolyzed by salivary amylase, artificial gastric juice, or pancreatic amylase, however they were hydrolyzed by enzymes of the small intestine.

Keywords

Optics and Photonics, Glycosylation, Magnetic Resonance Spectroscopy, Hydrolysis, Molecular Sequence Data, Oligosaccharides, Trehalose, Mass Spectrometry, Geobacillus stearothermophilus, Glucose, Carbohydrate Sequence, Glucosyltransferases, Glucan 1,4-alpha-Glucosidase, Chromatography, High Pressure Liquid

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