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Bioscience Biotechnology and Biochemistry
Article . 1996 . Peer-reviewed
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Purification and Characterization of a Lectin fromAmaranthus hypochondriacusvar. Mexico Seeds

Authors: OZEKI, Munetaka; KAMEMURA, Kazuo; MORIYAMA, Kikuko; ITOH, Yayoi; FURUICHI, Yukio; UMEKAWA, Hayato; TAKAHASHI, Takao;

Purification and Characterization of a Lectin fromAmaranthus hypochondriacusvar. Mexico Seeds

Abstract

A lectin from Amaranthus hypochondriacus var. Mexico (AHML) was purified by affinity chromatography using asialofetuin-Sepharose 4B. AHML is specific for N-acetyl-D-galactosamine as are the other Amaranthus lectins. AHML has no carbohydrate moiety and requires no metal ion for the hemagglutination activity. The pI of AHML is 6.8. AHML has a native molecular mass of 45.0 kDa and is composed of homo-subunits having molecular masses of 36.8 kDa.

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Keywords

Amaranthus, Erythrocytes, purification, Chemical Phenomena, Chemistry, Physical, In Vitro Techniques, Plants, Molecular Weight, Lectins, Seeds, lectin, Animals, Electrophoresis, Polyacrylamide Gel, hemagglutinin, Rabbits, Amino Acids, Isoelectric Focusing, Plant Lectins, Haptens

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    popularity
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    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
11
Top 10%
Top 10%
Top 10%