Isolation of Immortal Human Endothelial Cells
Copyright policy )Isolation of Immortal Human Endothelial Cells
To get immortalized endothelial cells, human endothelial cells were transfected with a temperature-sensitive simian virus 40 DNA. The majority of transfected cells showed a prolonged life span and temperature-dependent cell growth. One of the clones, #5-1, grew either at 33 or 37 degrees C and could be cultured more than 110 generations. The cells expressed several endothelial cell specific characteristics.
- Nagoya University Japan
Cell Survival, Heparin, Temperature, Genome, Viral, Simian virus 40, Immunohistochemistry, Lipoproteins, LDL, Blotting, Southern, Tissue Plasminogen Activator, Plasminogen Activator Inhibitor 1, Humans, Endothelium, Vascular, Cloning, Molecular, Antigens, Viral, Tumor, E-Selectin, Growth Substances, Cell Adhesion Molecules, Cell Division, Cells, Cultured, Plasmids
Cell Survival, Heparin, Temperature, Genome, Viral, Simian virus 40, Immunohistochemistry, Lipoproteins, LDL, Blotting, Southern, Tissue Plasminogen Activator, Plasminogen Activator Inhibitor 1, Humans, Endothelium, Vascular, Cloning, Molecular, Antigens, Viral, Tumor, E-Selectin, Growth Substances, Cell Adhesion Molecules, Cell Division, Cells, Cultured, Plasmids
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