The action of various enzymes on beet pulp was examined to increase the efficiency of enzymatic saccharification. Endogenous enzyme from the homogenate of sugar beet had higher activity for carboxymethyl-cellulose than for beet pulp. The enzyme from formerly isolated strain C had a similar action pattern to that of beet enzyme. The beet homogenate also contained pectin-methylesterase activity, which was clearly measured for the formation of free carboxyl groups in uronic acids by the fluorescent labeling method with water-soluble carbodiimide (EDC). Reduction of uronic acids with EDC–NaBH4 (Taylor method) stimulated the susceptibility of beet pulp to pectinase and led to the increase in the recovery of neutral sugar, glucose, and galactose. The limit of hydrolysis of beet pulp and EDC–NaBH4 reduced beet pulp was increased by the combination of pectinase and cellulase (Meicelase). These results gave useful information on the enzymatic conversion of beet pulp for ethanol fermentation by yeast.