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Bioscience Biotechnology and Biochemistry
Article . 1993 . Peer-reviewed
Full-Text: http://www.jstage.jst.go.jp/article/bbb1992/57/1/57_1_56/_pdf
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Bioscience Biotechnology and Biochemistry
Article
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Bioscience Biotechnology and Biochemistry
Article . 1993 . Peer-reviewed
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Bioscience Biotechnology and Biochemistry
Article . 1993
Data sources: Europe PubMed Central
https://dx.doi.org/10.1271/bbb...
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Enzymatic Synthesis of a Novel Trisaccharide, Glucosyl Lactoside

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1993 English Publisher:Informa UK LimitedJournal:Bioscience, Biotechnology, and Biochemistry, volume 57, pages 56-60 (issn: 0916-8451, eissn: 1347-6947, Copyright policy )
Authors: Shibuya, Takashi; Miwa, Yoshikatsu; Nakano, Masayuki; Yamauchi, Takako; Chaen, Hiroto; Sakai, Shuzo; Kurimoto, Masashi;

doi: 10.1271/bbb.57.56

pmid: 7763423

Enzymatic Synthesis of a Novel Trisaccharide, Glucosyl Lactoside

Abstract

Cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus produced a series of oligosaccharides by the transglycosylation reaction with cyclomaltohexaose as a glycosyl donor and D-lactose as its acceptor. The content of oligosaccharide A as the main transfer product was 22.1% of the total sugar. The content was increased to 34.1% by hydrolysis with glucoamylase. Oligosaccharide A was isolated by column chromatography and crystallized. By chemical and enzymatic analyses, oligosaccharide A was found to be a novel non-reducing trisaccharide; O-beta-D-galactopyranosyl-(1-->4)-O- beta-D-glucopyranosyl alpha-D-glucopyranoside.

Keywords

Cyclodextrins, alpha-Cyclodextrins, Glycosylation, Magnetic Resonance Spectroscopy, Hydrolysis, Molecular Sequence Data, Oligosaccharides, Lactose, Geobacillus stearothermophilus, Carbohydrate Sequence, Glucosyltransferases, Chromatography, Thin Layer, Crystallization, Trisaccharides, Chromatography, High Pressure Liquid

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    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 15
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Average
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
15
Average
Top 10%
Average
bronze