New Assays for Transketolase

Laurence Hecquet; Jean Bolte; Colette Demuynck

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Bioscience Biotechno...arrow_drop_down

Bioscience Biotechnology and Biochemistry

Article . 1993 . Peer-reviewed

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https://dx.doi.org/10.1271/bbb...

Article

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New Assays for Transketolase

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1993 English Publisher:Oxford University Press (OUP)Journal:Bioscience, Biotechnology, and Biochemistry, volume 57, pages 2,174-2,176 (issn: 0916-8451, eissn: 1347-6947,

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Authors: Laurence Hecquet; Jean Bolte; Colette Demuynck;

doi: 10.1271/bbb.57.2174

New Assays for Transketolase

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Abstract

Given the importance of transketolase, TK (EC 2.2.1.1) for both pharmacological studies and synthetic purposes, the need for a simple and inexpensive assay is patent. We describe here a simple and inexpensive TK assay using the unphosphorylated ketoses L-erythrulose, 4-deoxy-L-erythrulose, and 4-deoxy-D,L-erythrulose as donor substrates instead of D-xylulose-5-phosphate, with D-ribose-5-phosphate as the acceptor substrate.

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