Streptavidin-Aequorin Fusion Protein for Bioluminescent Immunoassay
Copyright policy )Streptavidin-Aequorin Fusion Protein for Bioluminescent Immunoassay
The fusion protein of streptavidin to aequorin (STA-AQ) was highly purified from inclusion bodies in Escherichia coli cells and applied to a bioluminescent sandwich immunoassay. α-Fetoprotein (AFP), which is a serological marker of liver cancer, was used as a model analyte to test STA-AQ in an immunoassay. The measurable range of AFP by the sandwich immunoassay, using the complex of STA-AQ and the biotinylated anti-AFP antibody, was 0.02-200 ng/mL with an average coefficient of variation of 4.9%. The detection sensitivity with the complex of STA-AQ and the biotinylated anti-AFP antibody was similar to that with the complex of biotinylated aequorin, streptavidin and the biotinylated anti-AFP antibody. STA-AQ would be a useful reporter protein for immunoassays.
Light, Recombinant Fusion Proteins, Gene Expression, inclusion bodies, Antibodies, fused protein, Aequorin, biotin, Limit of Detection, Biomarkers, Tumor, Escherichia coli, Humans, Biotinylation, photoprotein, Immunoassay, Liver Neoplasms, luciferase, Streptomyces, Luminescent Measurements, Calcium, Electrophoresis, Polyacrylamide Gel, Streptavidin, alpha-Fetoproteins
Light, Recombinant Fusion Proteins, Gene Expression, inclusion bodies, Antibodies, fused protein, Aequorin, biotin, Limit of Detection, Biomarkers, Tumor, Escherichia coli, Humans, Biotinylation, photoprotein, Immunoassay, Liver Neoplasms, luciferase, Streptomyces, Luminescent Measurements, Calcium, Electrophoresis, Polyacrylamide Gel, Streptavidin, alpha-Fetoproteins
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