The study conducted at Cagayan State University, Carig campus, Tuguegarao City, Cagayan, Philippines aimed to evaluate the health-related quality of life (HRQOL) among permanent faculty and administrative employees in 2023, examining its relationship with burnout, depression, and perceived stress levels. Utilizing measurements such as demographic, socioeconomic, employment, and health profiles, alongside assessments like the SF-36 questionnaire, abbreviated Maslach Burnout Inventory, Beck’s Depression Inventory, and the Perceived Stress Scale, 142 faculty and 29 administrative staff participated. Findings indicated that various aspects of respondents’ profiles influenced general health, stress, burnout, and depression levels. Poor physical functioning, role limitations due to physical and emotional problems, low vitality, emotional wellbeing, and social functioning issues, bodily pains, and overall poor health significantly correlated with heightened stress, burnout (including emotional exhaustion, depersonalization, and poor personal accomplishments), and severe depression. It was emphasized that even minor factors can impact employees’ overall wellbeing, underscoring the importance of effectively coping with stress, burnout, and depression to sustain high performance, motivation, satisfaction, and a sense of recognition, reward, and responsibility among employees. published by the International Journal of Biosciences | IJB