Crystallographic Studies of Mycobacterium tuberculosis Polyphosphate/ATP-NAD Kinase Complexed with NAD

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2004 English Publisher:Elsevier BVJournal:Journal of Bioscience and Bioengineering, volume 98, pages 391-393 (issn: 1389-1723, eissn: 1347-4421,

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Authors: Shigetarou, Mori; Masayuki, Yamasaki; Yukie, Maruyama; Keiko, Momma; Shigeyuki, Kawai; Wataru, Hashimoto; Bunzo, Mikami; +1 Authors

doi: 10.1263/jbb.98.391 , 10.1016/s1389-1723(04)00302-0

pmid: 16233726

Crystallographic Studies of Mycobacterium tuberculosis Polyphosphate/ATP-NAD Kinase Complexed with NAD

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Abstract

NAD kinase from Mycobacterium tuberculosis (Ppnk) uses ATP or inorganic polyphosphate [poly(P)]. Ppnk overexpressed in Escherichia coli was purified and crystallized in the presence of NAD. Preliminary X-ray analysis of the resultant crystal indicate that the crystal belongs to hexagonal space group P6(2)22 and is holo-Ppnk complexed with NAD.

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Kyoto University
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