Cloning and Expression of a NAD+-Dependent Xylitol Dehydrogenase Gene (xdhA) of Aspergillus oryzae

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2004 English Publisher:Elsevier BVJournal:Journal of Bioscience and Bioengineering, volume 97, pages 419-422 (issn: 1389-1723, eissn: 1347-4421,

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Authors: Lien Ha, Tran; Noriyuki, Kitamoto; Keiichi, Kawai; Kazuhiro, Takamizawa; Tohru, Suzuki;

doi: 10.1263/jbb.97.419 , 10.1016/s1389-1723(04)70229-7

pmid: 16233653

Cloning and Expression of a NAD+-Dependent Xylitol Dehydrogenase Gene (xdhA) of Aspergillus oryzae

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Abstract

XdhA, which encodes a xylitol dehydrogenase gene, was cloned from Aspergillus oryzae genomic DNA. It consists of 1214 bp structural region, which is interrupted by two introns, and encodes 358-amino-acid protein (38,197 Da). It is similar to the known NAD(+)-dependent xylitol dehydrogenase (EC 1.1.1.9). The gene was expressed in Escherichia coli BL21-AI using a T7 promoter. The cell-free extract of the transformant showed a 36.5 kDa band upon SDS-PAGE and NAD(+)-dependent xylitol dehydrogenase activity.

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