Overexpression of Aspergillus aculeatus Cellobiohydrolase I in Aspergillus oryzae

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2003 Japan English Publisher:Elsevier BVJournal:Journal of Bioscience and Bioengineering, volume 95, pages 627-629 (issn: 1389-1723, eissn: 1347-4421,

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Authors: Kanamasa, S.; Mochizuki, M.; Takada, G.; Kawaguchi, T.; Sumitani, J.; Arai, M.;

doi: 10.1263/jbb.95.627 , 10.1016/s1389-1723(03)80174-3

pmid: 16233469

Overexpression of Aspergillus aculeatus Cellobiohydrolase I in Aspergillus oryzae

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Abstract

To express the cbhI gene, encoding Aspergillus aculeatus cellobiohydrolase I (CBHI), in Aspergillus oryzae, a plasmid was constructed. The strain that displayed the strongest CBHI activity among the transformants produced about 941 mg/l in liquid culture. It was confirmed by a PCR method that the plasmid was integrated at the niaD locus.

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