
doi: 10.1263/jbb.102.564
pmid: 17270723
Aspergillus oryzae RIB40 niaD(-) was transformed using a plasmid constructed with the A. oryzae phytase gene and pNAN8142 vector. The culture broth of the transformant, which was grown in a medium containing starch as a carbon source and polyvinylpyrrolidone showed phytase activity of a maximum of 2.0 units ml(-1) at 37 degrees C, pH 5.5.
Enzyme Activation, 6-Phytase, Species Specificity, Aspergillus oryzae, Gene Expression, Plants, Genetically Modified, Protein Engineering, Recombinant Proteins
Enzyme Activation, 6-Phytase, Species Specificity, Aspergillus oryzae, Gene Expression, Plants, Genetically Modified, Protein Engineering, Recombinant Proteins
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