Analysis of Sugar Epimers Using Mass Spectrometry: N -Acetyllactosamine-6,6′-Disulfate and the 2′-Epimer
Copyright policy )Analysis of Sugar Epimers Using Mass Spectrometry: N -Acetyllactosamine-6,6′-Disulfate and the 2′-Epimer
Galβ1–4GlcNAc-6,6′-disulfate and 2′-epimer corresponding to Galβ1–4ManNAc-6,6′-disulfate were distinguished by mass spectrometry by utilizing fast atom bombardment (FAB), electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) methods. As for the steric information, negative-ion ESI mass spectrometry/mass spectrometry (MS/MS) provides the most extensive data, but FAB MS/MS also reveals detailed structural information of interest in our case, where MALDI MS is not yet fully equipped with post-source decay.
- University of Electro-Communications Japan
- Thermo Electron United States
- Kanagawa University Japan
Models, Molecular, Spectrometry, Mass, Electrospray Ionization, Carbohydrate Sequence, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Carbohydrate Conformation, Amino Sugars, Mass Spectrometry
Models, Molecular, Spectrometry, Mass, Electrospray Ionization, Carbohydrate Sequence, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Carbohydrate Conformation, Amino Sugars, Mass Spectrometry
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