A new flavanone glucoside, named andrographidine A (1), and five new flavone glucosides, named andrographidines B (2), C (3), D (4), E (5) and F (6), were isolated from the root of Andrographis paniculata. Their structures were determined on the basis of spectral data, especially carbon-13 and proton nuclear magnetic resonance spectra, and chemical derivatization. They have uncommon O-substitution patterns involving 5-, 7-, 8-, 2'-, 3'-and 4'-O-substituents.