
doi: 10.1248/bpb.26.361
pmid: 12612448
Molecular cloning of cDNA encoding a new member of the trypsinogen family, named trypsinogen hL, was carried out by PCR using human lung cDNAs as templates. The primary structure of trypsinogen hL was found to be a prepro-protein and a catalytic triad, 64His, 108Asp and 201Ser. It was also found that trypsinogen hL is specifically expressed in the human lung, the expression level being 30-times higher than those in other tissues tested. A phylogenic tree analysis showed that trypsinogen hL is a new member of the trypsinogen family, a family of serine protease family proteins.
DNA, Complementary, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Serine Endopeptidases, Gene Expression, Sequence Homology, Blotting, Northern, Evolution, Molecular, Trypsinogen, Humans, RNA, Messenger, Cloning, Molecular, Lung, Sequence Alignment
DNA, Complementary, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Serine Endopeptidases, Gene Expression, Sequence Homology, Blotting, Northern, Evolution, Molecular, Trypsinogen, Humans, RNA, Messenger, Cloning, Molecular, Lung, Sequence Alignment
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