Western Blotting for Ginseng Saponins, Ginsenosides Using Anti-ginsenoside Rb1 Monoclonal Antibody.

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1999 English Publisher:Pharmaceutical Society of JapanJournal:Biological and Pharmaceutical Bulletin, volume 22, pages 219-220 (issn: 0918-6158, eissn: 1347-5215,

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Authors: N, Fukuda; H, Tanaka; Y, Shoyama;

doi: 10.1248/bpb.22.219

pmid: 10077447

Western Blotting for Ginseng Saponins, Ginsenosides Using Anti-ginsenoside Rb1 Monoclonal Antibody.

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Abstract

Ginsenosides separated by silica gel TLC were blotted to a polyvinylidene difluoride (PVDF) membrane which was treated with a NaIO4 solution followed by bovine serum albumine (BSA), resulting in a ginsenoside-BSA conjugate on a PVDF membrane. The blotted bands were stained with monoclonal antibody (MAb). The newly established Western blotting method was used for the determination of ginsenosides and their distribution in various Panax species.

Related Organizations

Kyushu University
Japan

Keywords

Plants, Medicinal, Carbohydrate Sequence, Ginsenosides, Blotting, Western, Molecular Sequence Data, Antibodies, Monoclonal, Panax, Saponins

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