
doi: 10.1248/bpb.16.335
pmid: 8358380
Dermatan sulfate-proteoglycans (DS-PGs) were extracted from rabbit, rat and bovine defatted livers by magnesium chloride extraction and DEAE-cellulose chromatography, and then submitted successively to Asahipak GS-520 gel filtration chromatography, Asahipak ES-502N anion exchange chromatography, and cellulose acetate membrane electrophoresis. The disaccharide composition of the glycosaminoglycan chains was determined by differential digestion by chondroitinase ABC, AC, ACII and/or B followed by HPLC for analysis of the resulting unsaturated disaccharides. The hepatic dermatan sulfate chains contained disulfated disaccharide units; Di-diSB and Di-diSE. The hepatic DS-PGs were divided into two groups; Di-diSE-poor DS-PGs and Di-diSE-rich DS-PGs. The iduronic acid content of Di-diSE-poor dermatan sulfate chains was higher than that of Di-diSE-rich ones.
Chondroitin Sulfates, Magnesium Chloride, Dermatan Sulfate, Electrophoresis, Cellulose Acetate, Chromatography, Ion Exchange, Disaccharides, Chondroitinases and Chondroitin Lyases, Rats, Liver, Chromatography, Gel, Animals, Cattle, Rabbits, Chromatography, High Pressure Liquid
Chondroitin Sulfates, Magnesium Chloride, Dermatan Sulfate, Electrophoresis, Cellulose Acetate, Chromatography, Ion Exchange, Disaccharides, Chondroitinases and Chondroitin Lyases, Rats, Liver, Chromatography, Gel, Animals, Cattle, Rabbits, Chromatography, High Pressure Liquid
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