Mouse egg ribosomes: evidence for storage in lattices

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1981 English Publisher:The Company of BiologistsJournal:Development, volume 62, pages 153-164 (issn: 0950-1991, eissn: 1477-9129,

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Authors: R, Bachvarova; V, De Leon; I, Spiegelman;

doi: 10.1242/dev.62.1.153

pmid: 7196940

Mouse egg ribosomes: evidence for storage in lattices

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Abstract

ABSTRACT The ribosomes of mouse ova were labeled by exposure of growing ovarian oocytes to [3H]uridine. The ribosomes of ova lysed and dispersed in hypotonic medium were contained in particles with an unusually low buoyant density in CsCl density gradients. These particles sedimented at 9000g or less, and electron microscopy of the pellet revealed ribosome-like particles embedded in a fibrillar network. These results indicate that the ribosomes are present in a proteinaceous superstructure, probably the lattices seen in situ by transmission electron microscopy.

Related Organizations

Cornell University
United States

Keywords

Mice, Mice, Inbred ICR, Microscopy, Electron, Chlorides, Centrifugation, Density Gradient, Animals, Cesium, Female, Ribosomes, Ovum

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