ABSTRACT The Notch signaling pathway plays a crucial role in neurogenesis by regulating cell fate specification. However, its complexity poses challenges in uncovering the mechanisms underlying these decisions. This Review explores the intricacies of the Notch pathway, including its diverse activation mechanisms and the influence of post-translational modifications of Notch receptors and ligands on pathway outcomes. We discuss how Notch signaling regulates embryonic neurogenesis via interactions with proneural genes and with other signaling pathways. We also examine the role of Notch in adult neurogenesis, and the therapeutic potential of leveraging Notch signaling to reprogram glia in the adult brain. Lastly, we highlight emerging technologies that measure Notch dynamics and discuss remaining knowledge gaps. Together, these insights underscore the multifaceted role of Notch signaling and outline key directions for future research.