Systematic gain-of-function genetics in Drosophila
Copyright policy )Systematic gain-of-function genetics in Drosophila
ABSTRACT A modular misexpression system was used to carry out systematic gain-of-function genetic screens in Drosophila. The system is based on inducible expression of genes tagged by insertion of a P-element vector carrying a GAL4-regulated promoter oriented to transcribe flanking genomic sequences. To identify genes involved in eye and wing development, the 2300 independent lines were screened for dominant phenotypes. Among many novel genes, the screen identified known genes, including hedgehog and decapentaplegic, implicated in these processes. A genetic interaction screen for suppressors of a cell migration defect in a hypomorphic slow border cells mutant identified known genes with likely roles in tyrosine kinase signaling and control of actin cytoskeleton, among many novel genes. These studies demonstrate the ability of the modular misexpression system to identify developmentally important genes and suggest that it will be generally useful for genetic interaction screens.
- University of the West United States
- Howard Hughes Medical Institute United States
- Carnegie Institution for Science United States
- University of California, Berkeley United States
- European Molecular Biology Laboratory Germany
Male, Genome, Saccharomyces cerevisiae Proteins, Gene Expression Regulation, Developmental, Genes, Insect, Eye, DNA-Binding Proteins, Phenotype, Suppression, Genetic, Cell Movement, Mutation, CCAAT-Enhancer-Binding Proteins, Animals, Drosophila Proteins, Wings, Animal, Drosophila, Female, Genes, Dominant, Transcription Factors
Male, Genome, Saccharomyces cerevisiae Proteins, Gene Expression Regulation, Developmental, Genes, Insect, Eye, DNA-Binding Proteins, Phenotype, Suppression, Genetic, Cell Movement, Mutation, CCAAT-Enhancer-Binding Proteins, Animals, Drosophila Proteins, Wings, Animal, Drosophila, Female, Genes, Dominant, Transcription Factors
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