Functional and molecular characterization of the transcriptional regulatory region of Tcp-10bt, a testes-expressed gene from the t complex responder locus
Copyright policy )Functional and molecular characterization of the transcriptional regulatory region of Tcp-10bt, a testes-expressed gene from the t complex responder locus
ABSTRACT Mouse t haplotypes contain several mutant alleles that disrupt spermatogenesis. Their phenotypes include sterility, reduced fertility and transmission ratio distortion (TRD). The substantial genetic analyses of these mutant alleles, coupled with intensive physical characterization of the t complex, provides a fertile ground for identifying and understanding genes essential to male gametogenesis. The t complex responder (Tcr) locus plays a central role in this process, interacting with other t haplotype-encoded genes to mediate TRD. A candidate responder gene, Tcp-10bt, has been cloned and subjected to molecular characterization. Here, we define the transcriptional regulatory regions of this gene in transgenic mice. A 1.6 kb (but not 0.6 kb) DNA fragment upstream of the transcription start site contains all the regulatory signals for appropriate temporal and germ cell-specific expression of this gene. Two smaller fragments within this region bound specifically to nuclear factor(s) from germ cell protein extracts in gel shift assays. This work is a step towards understanding the mechanism of Tcp-10bt regulated expression and may ultimately help reveal a common regulatory pathway shared by other similarly expressed spermatogenic genes.
- Jackson Laboratory United States
- Cleveland Clinic Lerner College of Medicine United States
Male, 570, Electrophoresis-Polyacrylamide-Gel, Transcription, Genetic, Blotting-Northern, Ubiquitin-Protein Ligases, Molecular Sequence Data, Mice, Transgenic, Base-Sequence, Mice-Transgenic, 576, Mice, Testicular-Hormones: ge, SUPPORT-U-S-GOVT-P-H-S, Genes, Regulator, Testis, Animals, Spermatogenesis, Nuclear-Proteins: ge, SUPPORT-NON-U-S-GOVT, Genes-Regulator: ph, t-Complex Genome Region, Spermatogenesis: ge, Molecular-Sequence-Data, Base Sequence, Transcription-Genetic: ge, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Testis: ph, Blotting, Northern, Immunohistochemistry, DNA-Binding Proteins, Testicular Hormones, Electrophoresis, Polyacrylamide Gel, DNA-Binding-Proteins: ge, Microtubule-Associated Proteins
Male, 570, Electrophoresis-Polyacrylamide-Gel, Transcription, Genetic, Blotting-Northern, Ubiquitin-Protein Ligases, Molecular Sequence Data, Mice, Transgenic, Base-Sequence, Mice-Transgenic, 576, Mice, Testicular-Hormones: ge, SUPPORT-U-S-GOVT-P-H-S, Genes, Regulator, Testis, Animals, Spermatogenesis, Nuclear-Proteins: ge, SUPPORT-NON-U-S-GOVT, Genes-Regulator: ph, t-Complex Genome Region, Spermatogenesis: ge, Molecular-Sequence-Data, Base Sequence, Transcription-Genetic: ge, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Testis: ph, Blotting, Northern, Immunohistochemistry, DNA-Binding Proteins, Testicular Hormones, Electrophoresis, Polyacrylamide Gel, DNA-Binding-Proteins: ge, Microtubule-Associated Proteins
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).16 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!