Isolation and Purification of DNA

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Other literature type 12 Dec 2011 English Publisher:Informa UK Limited

Authors: Leland Cseke; Chuanfu An; Chung-Jui Tsai;

doi: 10.1201/b11351-3

Isolation and Purification of DNA

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Abstract

Deoxyribonucleic acid (DNA) isolation and purification are essential techniques for molecular biology studies. The quality and integrity of the DNA isolated directly affects the results of all subsequent scientific research.1–4 A number of methods have been developed for extraction and purification of DNA from different tissue types and organisms. We present several well-developed protocols here for the isolation of DNA from bacteria, lambda phages, animals, and plants and the purification of DNA fragments from agarose gels. CONTENTS

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