ENZYME SYSTEMS INVOLVED IN LIPID-MEDIATED SIGNALING Methods for Measuring the Activity and Expression of Phospholipases A2 Prerna Rastogi, Janhavi Sharma, and Jane McHowat Methods of Measuring the Activity and Expression of Phospholipases C Ryan A. Frieler, John A. Watt, and Thad A. Rosenberger Methods for Measuring the Activity and Expression of Phospholipase D Wenjuan Su and Michael A. Frohman Methods for Measuring the Activity and Expression of Diacylglycerol Kinase Kaoru Goto, Yasukazu Hozumi, Tomoyuki Nakano, Alberto M. Martelli, and Hisatake Kondo Methods for Measuring Endocannabinoid Production and Expression and Activity of Enzymes Involved in the Endocannabinoid System Tiziana Bisogno, Luciano De Petrocellis, and Vincenzo Di Marzo MASS SPECTROMETRY METHODS FOR LIPID ANALYSIS Mass Spectrometry Methods for the Analysis of Phospholipid Molecular Species Analysis: A Shotgun Lipidomics Approach Xianlin Han, Hua Cheng, Xuntian Jiang, and Youchun Zeng Mass Spectrometry Methods for the Analysis of Bioactive Sphingolipids: A High-Performance Liquid Chromatography/Tandem Mass Spectrometry Approach Jacek Bielawski, Motohiro Tani, and Yusuf A. Hannun Mass Spectrometry Methods for the Analysis of Oxidant Stress in Biological Fluids and Tissues: Quantification of F2-Isoprostanes and F4-Neuroprostanes Using Mass Spectrometry Ginger L. Milne and Jason D. Morrow LIPID-MEDIATED REGULATION OF GENE EXPRESSION Methods for Assessing the Role of Lipid-Activated Nuclear Receptors in the Regulation of Gene Transcription Jenny Kaeding, Jocelyn Trottier, and Olivier Barbier Fluorescence Methods to Assess the Impact of Lipid Binding Proteins on Ligand-Mediated Activation of Gene Expression Heather A. Hostetler, Avery L. McIntosh, Anca D. Petrescu, Huan Huang, Barbara P. Atshaves, Eric J. Murphy, Ann B. Kier, and Friedhelm Schroeder METHODS TO ASSESS FATTY ACID AND PHOSPHOLIPID METABOLISM Methods for Measuring Fatty Acids Using Chromatographic Techniques Gwendolyn Barcelo-Coblijn, Cameron C. Murphy, and Eric J. Murphy Methods for Measuring Fatty Acid Uptake and Targeting in Cultured Cells and In Vivo Paul G. Millner, Serena M. Lackman, and Eric J. Murphy Methods for Kinetic Analysis of Fatty Acid Incorporation and Turnover In Vivo: A Steady-State Kinetic Radiotracer Approach Dhaval P. Bhatt and Thad A. Rosenberger