A mainlobe interference suppression method is proposed in this paper, which can still work when the signal of interest (SOI) is present in the training data. In this method, the iterative adaptive approach (IAA) is applied to spatial spectrum estimation at first. Then, IAA spatial spectrum is used to reconstruct the interference-plus-noise covariance matrix (INCM). Next, the eigenvector associated with mainlobe interference in INCM is determined, and the eigen-projection matrix can be calculated to suppress the mainlobe interference. Meanwhile, the sidelobe-interference-plus-noise covariance matrix (SINCM) can be reconstructed. Finally, the adaptive weight vector is obtained. One main advantage is that the proposed method can deal with coherent mainlobe interference and sidelobe interferences simultaneously. Simulation results demonstrate the effectiveness and robustness of the proposed method.