Plaque Assay of Bovine Rotavirus

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 1983 English Publisher:Springer Science and Business Media LLCJournal:Acta Veterinaria Scandinavica, volume 24, pages 362-373 (eissn: 1751-0147,

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Authors: G, Butchaiah; E, Lund;

doi: 10.1186/bf03546710

pmid: 6326542

pmc: PMC8291256

Plaque Assay of Bovine Rotavirus

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Abstract

Incorporation of trypsin and diethylaminoethyl-dextran in the overlay was found to be necessary for infectivity assay of the UK strain of bovine rotavirus by plaque assays. Small plaques of about 1 mm in radius were formed in BGM cells. Large plaques of about 3–4 mm in radius were consistently produced in monolayers of secondary calf kidney cultures.

Related Organizations

University of Copenhagen
Denmark

Keywords

Rotavirus, Chlorocebus aethiops, Animals, Cattle, Viral Plaque Assay, Kidney, Cell Line

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