AbstractAn improvement in embedment and sectioning methodology of large tissue samples in glycol methacrylate resin is presented. The method improves efficiency by allowing relatively large tissue slabs, or multiple sets of smaller tissue samples to be embedded in large 2 × 3 inch blocks. The method described has general applicability for many histological procedures where large tissue sections are required. In the example presented here, the method also provides for relatively thick tissue sections to be generated (20–70 μm), thereby meeting the requirements of the optical dissector method for use with modern stereology procedures. The method's impact on increased efficiency for application of these stereological methods is discussed. (The J Histotechnol 26:49, 2003)Submitted: July 18, 2002; Accepted with revisions January 14, 2003