AN ANALYSIS OF FROZEN SECTION TECHNIQUES: I. SECTIONING OF FRESH-FROZEN TISSUE

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1957 English Publisher:SAGE PublicationsJournal:Journal of Histochemistry & Cytochemistry, volume 5, pages 47-52 (issn: 0022-1554, eissn: 1551-5044,

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Authors: W, THORNBURG; P E, MENGERS;

doi: 10.1177/5.1.47

pmid: 13416567

AN ANALYSIS OF FROZEN SECTION TECHNIQUES: I. SECTIONING OF FRESH-FROZEN TISSUE

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Abstract

Optimal Conditions for cutting thin sections from fresh-frozen tissue have been described in terms of a set of optimal temperatures for the tissue block, microtome chamber, and knife. A model for the cutting process is proposed which postulates a zone of "micro-melting" at the edge of the knife. The behavior of the freezing microtome is interpreted in terms of this model for a variety of conditions.

Related Organizations

University of Mary
United States

Keywords

Histocytological Preparation Techniques, Histology, Histological Techniques, Frozen Sections, Humans, Cryoultramicrotomy

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